Bus 40 - Americana Blvd/Universal Orlando: Full Schedule in Orlando

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      Backlot DR & Universal Studios Plz – N Garland AVE & W Amelia St

      Backlot DR & Universal Studios Plz

      05:2006:1506:4507:1507:4508:1508:4509:1509:4510:1510:4511:1511:4512:1512:4513:1513:4514:1514:4515:1515:4516:2016:5017:2017:5018:2019:2020:2021:2022:2023:1000:10 +1

      Vineland RD & Backlot Dr

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      Vineland RD & Cheryl St

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      Vineland RD & Universal Blvd

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      Universal Blvd & Hollywood Way

      04:4005:0305:2705:5706:2206:5207:2207:5208:2208:5209:2209:5210:2210:5211:2211:5212:2212:5213:2213:5214:2214:5215:2215:5216:2716:5717:2717:5718:2719:2720:2721:2722:2723:1700:17 +1

      S Kirkman RD & Windhover Dr

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      Conroy RD & Lawing Ln

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      Conroy RD & Pine Creek Dr

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      Conroy RD & Cypress Woods Dr

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      Conroy RD & Middlebrook Rd

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      Conroy RD & Southgate Dr

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      Conroy RD & Southgate Dr

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      Conroy RD & Emerald Forest Way

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      Conroy RD & Vineland Rd

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      Walden CIR & Vineland Rd

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      Walden CIR & Vineland Rd

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      Walden CIR & Vineland Rd

      04:5405:1905:4406:1406:3907:0907:3908:0908:3909:0909:3910:0910:3911:0911:3912:0912:3913:0913:3914:0914:3915:0915:3916:0916:4517:1517:4518:1518:4519:4420:4421:4422:4323:3300:33 +1

      Conroy RD & Millenia Blvd

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      Conroy RD & Water Garden Dr

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      Conroy RD & Water Garden Dr

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      Conroy RD & Eastgate Dr

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      Conroy RD & Moonglow Blvd

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      Conroy RD & Moxie Blvd

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      Conroy RD & Winter RUN Dr

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      Americana Blvd & Grand Central Pkwy

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      Americana Blvd & Arpana Dr

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      Americana Blvd & Texas Ave

      05:0305:2905:5506:2506:5007:2007:5008:2008:5109:2109:5110:2110:5111:2111:5212:2212:5213:2213:5214:2214:5215:2215:5216:2216:5817:2817:5818:2818:5819:5520:5521:5522:5423:4400:42 +1

      SAN Antonio AVE & Americana Blvd

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      SAN Antonio AVE & Lake Fountain Dr

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      SAN Antonio AVE & Honour Rd

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      Honour RD & S RIO Grande Ave

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      RIO Grande AVE S & Holden Ave

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      S RIO Grande AVE & Holden Ave

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      S RIO Grande AVE & 45th St

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      S RIO Grande AVE & 42nd St

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      S RIO Grande AVE & 40th St

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      S RIO Grande AVE & 37th St

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      S RIO Grande AVE & 34th St

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      S RIO Grande AVE & 30th St

      05:1205:3906:0506:3607:0107:3108:0108:3109:0209:3210:0210:3211:0211:3212:0312:3313:0313:3314:0314:3315:0315:3316:0316:3317:0817:3818:0818:3819:0720:0421:0422:0323:0223:5200:49 +1

      S RIO Grande AVE & 29th St

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      W Michigan ST & Recovery Way

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      W Michigan ST & S Orange Blossom Trl

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      W Michigan ST & Woods St

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      W Michigan ST & S Westmoreland Dr

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      W Michigan ST & Paseo Ave

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      W Michigan ST & Sunrise Ct

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      W Michigan ST & Taylor Ave

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      Orange AVE S & W Jersey St

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      S Orange AVE & E Crystal Lake St

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      S Orange AVE & E Muriel St

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      S Orange AVE & E Kaley St

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      S Orange AVE & E Hollenbeck St

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      W Miller ST & Kuhl Ave

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      W Miller ST & S Lucerne Ter

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      Sligh Blvd & W Copeland Dr

      05:2405:5306:1906:5107:1607:4608:1608:4609:1709:4710:1810:4811:1811:4812:1912:4913:1913:4914:1914:4915:1915:4916:1916:4917:2417:5418:2218:5219:1920:1521:1522:1423:1300:03 +100:59 +1

      Sligh Blvd & Columbia St

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      S Lucerne TER & W Gore St

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      W Gore ST & Sligh Blvd

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      W Gore ST & S Division Ave

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      S Division AVE & W Anderson St

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      S Division AVE AND W Anderson St

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      S Division AVE & W Church St

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      W Central Blvd & S Bryan Ave

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      N Garland AVE & W Central Blvd

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      N Garland AVE & W Washington St

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      N Garland AVE & W Amelia St

      05:3506:0506:3207:0407:3008:0008:3009:0009:3110:0110:3111:0111:3112:0112:3213:0213:3214:0214:3215:0215:3216:0216:3217:0217:3718:0718:3519:0519:3220:2721:2722:2623:2400:14 +101:09 +1
      Times marked with "—" indicate that the vehicle does not stop at this stop.
      Times marked with "?" indicate that the exact arrival time is unknown.
      The schedule is provided in the local timezone. Times with "(+1)" indicate departures on the next day.

      N Garland AVE & W Amelia St – Backlot DR & Universal Studios Plz

      N Garland AVE & W Amelia St04:0504:5005:2005:4506:1506:4507:1507:4508:1508:4509:1509:4510:1510:4511:1511:4512:1512:4513:1513:4514:1514:4515:1515:4516:1516:4517:1517:5018:2018:4519:4520:4521:4522:4523:45
      N Hughey AVE & W Robinson St???????????????????????????????????
      S Division AVE & W Central Blvd???????????????????????????????????
      S Division AVE & W Pine St???????????????????????????????????
      Division AVE & South St???????????????????????????????????
      S Division AVE & W Anderson St???????????????????????????????????
      S Division AVE & Carter St???????????????????????????????????
      S Division AVE & Callahan Dr???????????????????????????????????
      W Gore ST & S Hughey Ave???????????????????????????????????
      W Gore ST & Lucerne Ter???????????????????????????????????
      Lucerne TER & W Gore St???????????????????????????????????
      Lucerne TER & Columbia St???????????????????????????????????
      Sligh Blvd & W Copeland Dr04:1405:0105:3105:5706:2706:5807:2807:5808:2808:5809:2809:5810:2810:5811:2811:5812:2812:5813:2813:5814:2814:5915:2915:5916:2916:5917:2918:0418:3418:5919:5820:5821:5822:5723:56
      W Miller ST & Lucerne Ter???????????????????????????????????
      W Miller ST & Kuhl Ave???????????????????????????????????
      S Orange AVE & Lake Beauty Dr???????????????????????????????????
      S Orange AVE & Pennsylvania St???????????????????????????????????
      S Orange AVE & W Harding St???????????????????????????????????
      S Orange AVE & Grant St???????????????????????????????????
      W Michigan ST & Kunze Ave???????????????????????????????????
      W Michigan ST & Lake Holden Ter???????????????????????????????????
      W Michigan ST & Alamo Dr???????????????????????????????????
      W Michigan ST & S Westmoreland Dr???????????????????????????????????
      W Michigan ST & Woods Ave???????????????????????????????????
      W Michigan ST & S Orange Blossom Trl???????????????????????????????????
      W Michigan ST & S Orange Blossom Trl???????????????????????????????????
      W Michigan ST & Recovery Way04:2405:1305:4306:0906:3907:1207:4208:1208:4209:1209:4210:1310:4311:1311:4312:1312:4313:1313:4314:1314:4315:1415:4416:1416:4417:1317:4318:1718:4719:1120:1021:1022:1023:0800:06 +1
      S RIO Grande AVE & 30th St???????????????????????????????????
      S RIO Grande AVE & 34th St???????????????????????????????????
      S RIO Grande AVE & 37th St???????????????????????????????????
      S RIO Grande AVE & 40th St???????????????????????????????????
      S RIO Grande AVE & 42nd St???????????????????????????????????
      S RIO Grande AVE & 44th St???????????????????????????????????
      S RIO Grande AVE & Holden Ave???????????????????????????????????
      RIO Grande AVE S & Holden Ave???????????????????????????????????
      S RIO Grande AVE & Honour Rd???????????????????????????????????
      Honour RD & Grande Pointe Blvd???????????????????????????????????
      SAN Antonio AVE & Honour Rd???????????????????????????????????
      SAN Antonio AVE & Lake Fountain Dr???????????????????????????????????
      SAN Antonio AVE & Americana Blvd???????????????????????????????????
      Americana Blvd & Texas Ave04:3205:2305:5306:2006:5007:2307:5308:2308:5309:2409:5410:2510:5511:2611:5612:2612:5613:2613:5614:2614:5615:2715:5716:2716:5717:2417:5418:2818:5819:2220:2121:2122:2023:1800:15 +1
      Americana Blvd & Arpana Dr???????????????????????????????????
      Americana Blvd & Grand Central Pkwy???????????????????????????????????
      Conroy RD & S John Young Pkwy???????????????????????????????????
      Conroy RD & Winter RUN Dr???????????????????????????????????
      Conroy RD & Tradition Pkwy???????????????????????????????????
      Conroy RD & Moonglow Blvd???????????????????????????????????
      Conroy RD & Eastgate Dr???????????????????????????????????
      Conroy RD & Water Garden Dr???????????????????????????????????
      Conroy RD & Millenia Blvd???????????????????????????????????
      Walden CIR & Vineland Rd???????????????????????????????????
      Walden CIR & Vineland Rd???????????????????????????????????
      Walden CIR & Vineland Rd04:4305:3606:0606:3407:0407:3708:0708:3809:0809:3910:0910:4111:1111:4212:1212:4213:1213:4314:1314:4415:1415:4516:1516:4417:1417:4118:1118:4419:1419:3820:3621:3522:3423:3000:26 +1
      Conroy RD & Vineland Rd???????????????????????????????????
      Conroy RD & Emerald Forest Way???????????????????????????????????
      Conroy RD & Cason Cove Dr???????????????????????????????????
      Conroy RD & Pres Barack Obama Pkwy???????????????????????????????????
      Conroy RD & Middlebrook Rd???????????????????????????????????
      Conroy RD & Middlebrook Rd???????????????????????????????????
      Conroy RD & Pine Creek Dr???????????????????????????????????
      S Kirkman RD & Conroy Rd???????????????????????????????????
      S Kirkman RD & Windhover Dr???????????????????????????????????
      S Kirkman RD & Major Blvd???????????????????????????????????
      Universal Blvd & Hollywood Way04:5605:5106:2106:5107:2107:5508:2508:5609:2609:5710:2710:5911:2912:0012:3013:0013:3014:0114:3115:0215:3216:0316:3317:0217:3217:5918:2919:0119:3119:5520:5221:5122:4923:4400:39 +1
      Vineland RD & Peregrine Ave???????????????????????????????????
      Vineland RD & Cheryl St???????????????????????????????????
      Backlot DR & Universal Studios Plz05:0305:5806:2806:5807:2808:0208:3209:0309:3310:0410:3411:0611:3612:0712:3713:0713:3714:0814:3815:0915:3916:1016:4017:0917:3918:0618:3619:0819:3820:0220:5921:5822:5623:5100:46 +1
      Times marked with "—" indicate that the vehicle does not stop at this stop.
      Times marked with "?" indicate that the exact arrival time is unknown.
      The schedule is provided in the local timezone. Times with "(+1)" indicate departures on the next day.
      Times marked with "—" indicate that the vehicle does not stop at this stop.
      Times marked with "?" indicate that the exact arrival time is unknown.
      The schedule is provided in the local timezone. Times with "(+1)" indicate departures on the next day.
      Last updated on March 25, 2026
      Build real-time departures into your app Free API Access →

      Route Path (Streets & Roads)

      Outbound: Backlot DR & Universal Studios Plz – N Garland AVE & W Amelia St
      Vineland Road - Kirkman Road - Conroy Road - Walden Circle - Conroy Road - Americana Boulevard - San Antonio Avenue - Honour Road - Rio Grande Avenue - Michigan Street - South Orange Avenue - West Miller Street - Sligh Boulevard - Lucerne Terrace - West Gore Street - South Division Avenue - West Central Boulevard - North Garland Avenue - West Amelia Street
      Inbound: N Garland AVE & W Amelia St – Backlot DR & Universal Studios Plz
      West Amelia Street - North Hughey Avenue - South Division Avenue - East-West Expressway - West Gore Street - Lucerne Terrace - Sligh Boulevard - West Miller Street - South Orange Avenue - Pennsylvania Street - South Orange Avenue - Michigan Street - Rio Grande Avenue - Honour Road - San Antonio Avenue - Americana Boulevard - Conroy Road - Walden Circle - Conroy Road - Kirkman Road - Vineland Road
      All data is available in GTFS feed: collier-area-transit

      Bus 40 FAQ

      • What time does the 40 bus start operating?

        Services on the 40 - Americana Blvd/Universal Orlando bus start at 04:40 on Monday, Tuesday, Wednesday, Thursday, Friday, Saturday, Sunday.

      • What time does the 40 bus stop working?

        Services on the 40 - Americana Blvd/Universal Orlando bus stop at 01:09 +1 on Monday, Tuesday, Wednesday, Thursday, Friday, Saturday, Sunday.

      • Is there a 40 bus stop near me?

        Click here to view the nearest 40 - Americana Blvd/Universal Orlando bus stop.

      40 (Orlando Buses)

      The first stop of the 40 bus route is Backlot DR & Universal Studios Plz and the last stop is N Garland AVE & W Amelia St. 40 (Americana Blvd/Universal Orlando) is operational during everyday.

      Additional information: 40 has 66 stops and the total trip duration for this route is approximately 77 minutes.

      See why over 2 million users trust and consider busmaps.com as the best public transport website. busmaps gives you Orlando Buses suggested routes, real-time bus tracker, line route maps in Orlando, and helps to find the closest 40 bus stops near you. No internet available? Download an offline PDF map and bus schedule for the 40 bus to take on your trip.

      40 bus near me