Cities of Lee County
- Bonita Springs (2)
- Cape Coral (4)
- Cypress Lake (2)
- Estero (5)
- Fort Myers (28)
- Fort Myers Beach (3)
- Fort Myers Shores (1)
- Harlem Heights (1)
- Lehigh Acres (1)
- Lochmoor Waterway Estates (1)
- McGregor (1)
- North Fort Myers (1)
- Olga (1)
- Page Park (1)
- Palmona Park (3)
- Pine Manor (2)
- San Carlos Park (1)
- Suncoast Estates (1)
- Three Oaks (3)
- Tice (2)
- Villas (5)
The number in parentheses indicates the number of routes.